Science Homework Help
Science Homework Help. structural biology-Design an experiment using SDS-PAGE to determine the molecular size of the protein 4PN6
I have completed part A. will send writer the part A but question should not be relevant and could be separated.
B)Design an experiment using SDS-PAGE to determine the molecular size of the protein.
a.Present your experiment as a flow chart and indicate the rationale behind each step besides the flow chart.
b.Draw a gel diagram to indicate the expected size of the protein.
Some guiding questions:
1)Are there any inter/intra molecular disulphide bonds in the protein?
2)Are there any other post-translational modification to the protein that may affect the size of the protein?
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