Science Homework Help

Submit a PDF that contains your data from your SDS-PAGE experiment; be sure to include all data sets, your interpretation/explanation of your data, overall conclusion and references.

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Data Analysis (100 points total):

A) Presentation of Data/Results (20 points total)

• Include all actual raw data sets from your experiment. Examples include charts, graphs, gel pictures, blots, and/or fluorescence images. Properly label all data sets. (20 points)

Make sure to title each piece of data and include proper labels or identification keys.

Your 20 points for data sets will be graded as follows:

• 16-20 pts. – All results are presented, clearly labeled, and separated into sub-areas with detailed and accurate data. It

B) Explanation of Data/Results (30 points total)

• Text description of obtained results. (20 points total)
  • List two pairs of primary and secondary antibodies that you can use in this experiment. Explain your reasoning for your choices. (10 points)
  • What best explains the difference in the intensity of the 80kDa STAT3 bands seen in HepG2 and C2C12? (5 points)
  • What best explains the observation of bands between 135kD and 260kD in the HepG2 and K562 lanes? (5 points)
• Explanation of obtained results. Why did you get these results? Use scientific reasons to explain all of your data. (10 points)

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C) Discussion (40 points total)

• What was the goal of this experiment? (5 points)
• Write a brief summary about what you can conclude from the data/results. (5 points)
• If you repeated this experiment, how would you incorporate a positive and a negative control into your results? (5 points for positive control, 5 points for negative control; 10 points total)
• Explain why STAT3 has different expression levels in different cell lines. (5 points)
• Briefly described how antibodies are used as a therapeutic for COVID-19. (5 points)
• Describe how antibody tests for COVID-19 work. (5 points)
• Briefly describe your next experiment and the questions that it will answer about your protein sample. (5 points)

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D) In text citations (5 points total)

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E) References page (5 points total)