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Figure 2. Effect of High Cholesterol

Diet on Plasma Cholesterol Concentration.

Finger-prick blood samples were centrifuged at 1200 g for 1 min and plasma samples were collected and incubated with reaction mixture (0.1 M Tris-HCl, 1 mM 4-aminoantipyrine, 60 mM phenol, 1 mM sodium cholate, 280 U peroxidase, 7 mM Triton X-100, 65 U cholesterol esterase, 45 U cholesterol oxidase, pH7.7) for 10 min at room temperature. The absorbency of resulting solution was determined at 500 nm.

Figure 3. 1010Subject 18Subject 2cholesterol (mM)Subject 3cholesterol (mM)Subject 46Subject 1Subject 2Subject 3Subject 46240O6time (weeks)1230612time (weeks)